■ 基本信息
別名: | pGBKT7-BD |
啟動(dòng)子: | ADH1 |
復(fù)制子: | 2μ |
質(zhì)粒分類: | 酵母系列，酵母雙雜交載體 |
質(zhì)粒大小: | 7303bp |
原核抗性: | Kan |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 酵母細(xì)胞 |
備注: | 酵母雙雜交空載體 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 雜交 |
片段類型: | ORF |
片段物種: | 空載體 |
原核抗性: | Kan |
真核抗性: | TRP1
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The pEXT03/BD (pGBKT7/BD) vector expresses proteins fused to amino acids 1–147 of the GAL4 DNA binding domain (DNA-BD) for constructing fusion proteins with a bait protein. pGBKT7 replicates autonomously in both E. coli and S.cerevisiae from the pUC and 2 μ ori, respectivel.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK979pGBKT7 （別名 pGBKT7-BD）酵母雙雜交質(zhì)粒.txt
