■ 基本信息
復(fù)制子: | pUC |
質(zhì)粒分類: | 酵母系列質(zhì)粒；酵母表達(dá)質(zhì)粒；釀酒酵母質(zhì)粒 |
質(zhì)粒大小: | 4456bp |
原核抗性: | Amp |
真核抗性: | HIS3 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | YPH499，YPH500等釀酒酵母 |
培養(yǎng)條件: | 30℃，YPD，有氧 |
5'測(cè)序引物: | T7(TAATACGACTCACTATAGGG) |
3'測(cè)序引物: | M13R(CAGGAAACAGCTATGACC) |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | HIS3
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pRS4xx系列質(zhì)粒被用于基因的穿梭載體克隆釀酒酵母，但能夠在大腸桿菌中復(fù)制。雖然在大腸桿菌中，他們從pMB1的復(fù)制原點(diǎn)復(fù)制至pBR322。他們攜帶的bla（APR）標(biāo)記用于選擇用氨芐青霉素和復(fù)制的噬菌體F1原點(diǎn)單鏈DNA 。pRS403 through pRS406 are integrative plasmids (YIp); pRS413through pRS416 (shown below) are centromeric plasmids (YCp),and differ from the YIp by the insertion of a centromere (CEN)and autonomously replicating sequence (ARS); pRS423 throughpRS426 are episomal plasmids (YEp), and carry instead theorigin of replication from the yeast 2μ circle plasmid along withREP3 and FRT sequences (2). The 4 vectors in each series differonly in the yeast auxotrophic marker used: HIS3 (pRS4x3), TRP1(pRS4x4), LEU2 (pRS4x5) or URA3 (pRS4x6).
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK959pRS403釀酒酵母質(zhì)粒.txt
